IDENTIFICACIÓN DE MARCADORES MOLECULARES MICROSATÉLITES (SSR) PARA ESTUDIOS GENÉTICOS EN ANNONA DECEPTRIX WESTRA H RAINER, ESPECIE AMENAZADA EN LA PROVINCIA DE MANABÍ

Abstract

IN THE PROVINCE OF MANABÍ, THERE ARE A GREAT VARIETY OF SPECIES OF THE ANNONACEAE FAMILY THAT ARE IN A CERTAIN DEGREE OF VULNERABILITY, AMONG THEM IS ANNONA MANABIENSIS SAFF. EX R.E. FR, ANNONA CONICA RUIZ & PAV. EX G. DON AND ONE OF THE MOST IMPORTANT IN THIS STUDY ANNONA DECEPTRIX WESTRA H. RAINER, WHERE ITS FRUITS HAVE AMAZING CHARACTERISTICS RELATED IN (SHAPE, SIZE AND COLOR) AND THAT CAN BE CONSIDERED VERY IMPORTANT IN THE DIET OF THE FOR THIS REASON, THE OBJECTIVE OF THIS WORK WAS TO DEVELOP SSR MICROSATELLITE MARKERS IN A. DECEPTRIX AND THEIR APPLICATION IN OTHER ANNONACEAE FOR GENETIC STUDIES, AND THEN PROCEEDED TO PERFORM DNA EXTRACTION IN YOUNG SHOOT LEAVES WITH THE CTAB EXTRACTION PROTOCOL ( CETYLRIMELAMMONIUM BROMIDE) (DOYLE & DOYLE, 1990), FROM THE EXTRACTION, GENOMIC LIBRARIES WERE FORMED USING THE NEXTERA PLATFORM, CONTINUING WITH THE IDENTIFICATION OF MICROSATELLITES USING THE MSATCOMANDER SOFTWARE, THE DEVELOPMENT OF THE MARKERS ALLOWED OBTAINING VERY RELEVANT INFORMATION REGARDING TO ITS GENETIC CONTENT, AS IT WAS IN THE ESTIMATION OF GENETIC DIVERSITY BY LOCUS THAT PROVIDED A PRO AVERAGE IN THE NUMBER OF ALLELES (NA) OF 3,481, WITH A HIGHER VALUE IN THE AD8 AND AD11 LOCUS WITH 6 AND 5.6 RESPECTIVELY