Facultad de Ciencias Veterinarias

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Browsing Facultad de Ciencias Veterinarias by Author "Acosta Meza, Iris Cicibel"
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    " Efecto de la Harina de Cáscara de Mango (Mangifera indica L) sobre la Obesidad abdominal, Perfil lipídico y Morfometría del epitelio intestinal en ratones CD1.
    (Facultad de Ciencias Veterinarias > Carrera de Medicina Veterinaria, 2019) Acosta Meza, Iris Cicibel ; Vèlez Echeverria, Gema Genith
    The objective of this study was to evaluate the HCM effect (mango peel flour, Mangifera indica L) on abdominal obesity, lipid profile and morphometry of the intestinal epithelium. The experiment was carried out in the Agricultural Laboratories of the Technical University of Manabí in 28 male CD1 mice, weighing 25 to 30 grams. The mice were randomly distributed and received the food corresponding to the following treatments Conventional (T1), conventional + 4% HCM (T2), hyper fat (T3) and hyper fat + 4% HCM (T4) diets for 16 consecutive weeks. After the experimental period, the mice were previously sacrificed overnight fasting, with an intraperitoneal (IP) injection of a mixture of xylazine (25 mg / Kg) and ketamine (150 mg / Kg), blood samples were obtained by exanguination (1 to 1.5 ml), which are used for the analysis of the lipid profile and blood glucose by spectrophotometry. Abdominal fat, liver and heart were extracted from each experimental unit and the relative weight of these organs was calculated. In addition, jejunum and duodenal samples will be taken, these samples were preserved in 10% formalin of general staining of hematoxylin and eosin in paraffin sections. The results were analyzed by Analysis of Variance (ANOVA), Completely Random Design (DCA) with 2x2 factorial arrangement and the Tukey mean comparison test (p <0.05). The results obtained were: higher food consumption in T1, while the highest caloric intake, abdominal fat deposition and final weight were detected in the treatments that received a hyper fat diet (p <0.05). The relative weight of the liver is not different between treatments (p> 0.05), while the relative weight of the heart is lower in T3 in relation to the remaining treatments (p <0.05). In addition, the serum concentration of glucose, total cholesterol, triacylglycerol, LDL and the HDL / LDL index was not different between treatments (p> 0.05). Additionally, the serum concentration of glucose, total cholesterol, triacylglycerol, LDL and the HDL / LDL index was not different between treatments (p> 0.05). However, a higher concentration of HDL in T4 (p <0.05) was reported; the CT / HDL ratio (atherosclerotic index) tended to be lower (0.072) in diets supplemented with HCM (T2 and T4). Modification in intestinal morphometry (length, width and depth of crypts) is not modified. In conclusion, these results suggest that HCM could influence the lipid profile of mice, without modifying somatic parameters. Keywords: Abdominal fat, mango peel flour, obesity, polyphenols, CD1 mice.