"Efecto de la suplementación con harina de cáscara de mango (Mangifera indica L.) sobre el estado nutricional y hepático de ratones CD-1 inducidos a obesidad"

Abstract

The present investigation evaluated the effect of supplementation whit handle peel flour (HCM) on the nutritional and liver status of CD-1 mice induced to obesity. For this, during 8 weeks 36 biomodels were randomly distributed in two treatments: control diet (c) and hyperlipidic diet (HL). Subsequently, the mice were randomly distributed, depending on the suplementation of diets C and HL whit different doses of HCM (0%, 4% and 8%), in the following treatments: T1: control diet (C), T2: C+4% HCM, T3: C+8% HCM, T4: HL, T5: HL+4% HCM, T6: HL+8% HCM, for four weeks. The mice were subsequently sacrificed after a 12-hour fast. The weight, the naso-anal length of the mice, the food consumption, the relative weight of the liver, páncreas, kidneys, heart and abdominal fat, the serum concentration of cholesterol, and the enzymatic levels of alanine transaminase (ALT) and aspartate transaminase (ast). The results of the first stage were analyzed using the student T test, in the second stage an ANOVA analysis of variance was used, a completely randomized desing whit fatorial arrangement (3x2) or the non-parametric krustal-Wallis test, in the case of ANOVA requirements not begin met. Food consumption was significantly higher in the treatments that included the C diet tan in the treatments that included the HL diet. Likewise, it was observed that the mice that received the C diet (p<0,001). In the remaining zoometric parameters, there were no differences between treatments as a result of HCM supplementation. The weight difference was not a product of HCM intake, on the other hand; if there is a difference for the length, relative weight of kidneys and adominal fat. The hyperlipidic diet increased the plasma values of total cholesterol p: 0.014 (non-parametric Krustal-Wallis test), the serum cncentration of ALT and AST and the AST/ALT ratio didn´t differ between treatments. Thus, HCM didn´t generate negative effects in mice.