Germinación y establecimiento in vitro de Morella sp. (Myricaceae) especie arbórea multipropósito del Área Protegida Comunitaria Tambillo

Abstract

Laurel de cera (Morella sp.) is a forest tree species of ecological, industrial and medicinal importance, however, presents difficulties in its propagation due to the low percentages in the germination of the seeds. The objective of this research was to evaluate the effect of GA3 on seed germination and in vitro establishment of Morella sp. apices from the Tambillo Community Protected Area. The seeds were subjected to pre-germinative treatments with GA3 at concentrations of 250 ppm and 500 ppm for 24 and 48 h and were sown in germination trays with peat as substrate. For in vitro establishment the explants were placed in a solution of sodium hypochlorite (NaOCl) and quaternary ammonium (QA) in three concentrations (1, 1.5 and 2%) and three immersion times (5, 10 and 15 min) and were seeded in the Woody Plant Medium (WPM) formulation. The percentage of germination, percentage of contamination and percentage of dead explants were evaluated. The highest germination percentages were obtained with 500 ppm of GA3 in an immersion time of 24 and 48 h. The lowest percentage of contamination and of dead explants was achieved with 1.5% QA for 10 min.