"Lesiones renales asociadas a leptospirosis bovina y su relación con los títulos de anticuerpos presentes en animales destinados al sacrificio"

Abstract

In order to correlate kidney lesions associated with bovine leptospirosis with the presence of circulating antibodies against this disease and the types of serovars identified in slaughter animals, the kidneys of 277 cattle were inspected at the slaughterhouse in Manta canton, which receives animals from different cantons in Manabí province (Chone, El Carmen, Tosagua, Sucre, Jama, Bolívar and Junin). For the macroscopic interpretation of the lesions, the presence of inflammatory and degenerative processes compatible with bovine leptospirosis (interstitial nephritis and nephrosis), among other macroscopic alterations, were taken into consideration. Samples were taken from 100% of the lesioned organs for the histopathological study those that were processed by the classic technique of inclusion and cuts in paraffin blocks, were stained with Hematoxylin and Eosin. Serum samples from all animals examined were processed and analyzed using the Microscopic Agglutination Technique (MAT), to determine the antibody titles (Ac.) against interrogans leptospira and to demonstrate the serovars present. The results were statistically evaluated using the comparison test of multiple proportions to consider the number of kidneys affected in relation to the total number of animals examined and the interrelationship was established between the number of kidneys affected with lesions compatible with the disease and the presence of specific (Ac.) and the different serovars. In the macroscopic examination, it was found that 23.4% of the slaughtered animals had renal lesions and the most commonly found lesion was interstitial nephritis (61.5%). From the microscopic point of view, tubulo-interstitial nephropathy was observed (39.6%). 100% of cattle with nephritis and nephrosis were seroreactors. The serovars present in all the cantons, in these animals were Icterohaemorrhagiae, Hardjo and Pomona, the Chone Canton showed significant differences (p <0.05) in relation to the rest presenting 41.5% of affected kidneys.