"Lesiones renales en cerdos de matadero compatibles con leptospirosis porcina"

Abstract

In order to establish the relationship of renal lesions found in slaughterhouse pigs, with a positive diagnosis of porcine leptospirosis through molecular studies by PCR, 303 pigs slaughtered in the Rocafuerte slaughterhouse were examined macroscopically, kidney samples were taken from all of them to PCR analysis and samples were taken for the histopathological study of the cases that were positive. The macro and microscopic alterations were classified in those that from the etiopathogenic point of view in alterations related to porcine leptospirosis (ARL) which include inflammatory processes of the interstitium (interstitial nephritis), described as the classic kidney with white spots or streaks and alterations not directly related to porcine leptospirosis (NRL) that include the degenerative or necrotic processes of the renal tubules (nephrosis), circulatory disorders and cystic processes, among others. For the identification of pathogenic leptospira in the samples under study, the real-time Polymerase Chain Reaction technique coding for the LipL32 gene according to Stoddard, (2013) was used. For the statistical analysis of the samples, the Multiple Proportion Comparison test was used based on the COMPAPROWIN_2.0.1 Program, and the Chi Square test using the SPSS version 21 statistical package. The morphopathological alteration that was most frequently observed in the 303 animals was interstitial nephritis (54.2%) showing significant differences (p <0.05%) in relation to the rest of the lesions found. Of the total cases examined, 17.4% (53 pigs) were positive for leptospirosis by PCR, of which 33 animals presented various renal lesions, 87.8% represented (29 cases) presented (ARL) showing statistical significance (P <0.05) unlike the (ANRL), the analysis of the results by means of the Chi-square test, shows high statistical XV significance among the analyzed variables. (P <0.001) which suggests a high dependence on nephritis with the presence of leptospira. Keywords: renal lesions, leptospirosis, histopathology, polymerase chain reaction, morphopathological alteration, interstitial nephritis