Respuesta de tres especies de Citrus a fitorreguladores de crecimiento en la propagación in vitro

Abstract

THE CITRUS GENUS IS CULTIVATED IN MORE THAN 140 COUNTRIES, MAINLY IN TROPICAL AND SUBTROPICAL AREAS, REPRESENTING ONE OF THE MOST IMPORTANT FRUIT CROPS, IN TERMS OF ECONOMIC VALUE AND HUMAN NUTRITION. IN VITRO TISSUE CULTURE IS A TOOL THAT ALLOWS THE RELIABLE PROPAGATION OF LARGE VOLUMES OF PLANTS IN LESS TIME. THE OBJECTIVE OF THE RESEARCH WAS TO EVALUATE THE EFFECT OF GROWTH REGULATORS ON THE IN VITRO PROPAGATION OF THREE CITRUS SPECIES (C. SINENSIS, C. RETICULATA, C. AURANTIIFOLIA). A COMPLETELY RANDOM DESIGN (DCA) WAS ESTABLISHED IN FACTORIAL ARRANGEMENT (AXBXC)+1, APPLYING TUKEY'S MULTIPLE COMPARISON TEST (P <0.05) FOR THE MEANS OF THE TREATMENTS. THE MICROPROPAGATION WAS CARRIED OUT IN THREE PHASES: 1) ESTABLISHMENT PHASE: THE SEEDS OF EACH SPECIES WERE PLACED IN MURASHIGE AND SKOOG (MS) CULTURE MEDIUM AND THE DAYS TO GERMINATION AND CONTAMINATION (%) WERE EVALUATED; 2) MULTIPLICATION PHASE: THE APICES OF SEVEN-WEEK-OLD SEEDLINGS, FROM THE ESTABLISHMENT, WITH DIFFERENT CONCENTRATIONS (0.5 MG L; 0.75 MG L; 1 MG L-1) OF N-6 BENZYLAMINOPURINE (BAP) AND KINETIN (KIN), FROM WHICH THE NUMBER OF SHOOTS, LENGTH OF SHOOTS (CM) AND DIAMETER OF SHOOTS (MM) WERE EVALUATED; AND 3) ROOTING PHASE: THE SEEDLING EXPLANTS FROM THE MULTIPLICATION WERE SUBJECTED TO DIFFERENT LEVELS (0.5 MG L; 0.75 MG L; 1 MG L-1) OF NAPHTHALENEACETIC ACID (ANA) AND INDOLEACETIC ACID ( IAA), THE PRESENCE OF ROOTS, NUMBER OF ROOTS AND LENGTH OF ROOTS WERE EVALUATED