"Implementaciòn de equipos para la realizaciòn de la tècnica de reacciòn en cadena de la polimerasa en el laboratorio de Leptospira de la Facultad de Ciencias Veterinarias".

Abstract

The present work in the Community Development modality consisted of the implementation of equipment for the leptospira laboratory that is located in the Santa Ana cantón, Lodana parish in the Veterinary Sciences laboratory of the Technical University of Manabí, the project had as purpose to provide laboratory equipment to perform molecular studies through conventional Polymerase Chain Reaction (PCR) with greater sensitivity to diagnose pathogens of clinical importance. Leptospirosis is considered a worldwide zoonosis that is transmitted to humans by contact with infected animals, so it is necessary to identify and diagnose it using techniques with greater sensitivity and specificity, which is accomplished by means of the PCR technique. Due to the lack of such equipment in the area of leptospira, the realization of the project was put into practice; in addition to that it served for other undergraduate and graduate students to initiate research projects in this area. A thermocycler for 96 samples and an electrophoresis chamber with an external source were purchased, making it possible to advance in molecular techniques in this area of leptospira. In addition, it was possible to carry out a practice with the equipment from 20 DNA samples from bovines of the animal production department, obtaining negative results in the analyzed samples, which allowed knowing its operation.