"Funciòn hepàtica de pollos de negorde criados en ambiente tropical y suplementos con harina de tomate Solanum Licopersicum".

Abstract

In order to evaluate the effect of tomato meal (HT) supplementation on the liver function of broilers, an investigation was carried out with 300 one-day-old Cobb 500 chicks with an average weight of 48.8 ± 2.6 g, which were fed a conventional diet that covered their nutritional requirements; At 28 days of age, they were randomly distributed in (T1, T2, T3, T4, T5 and T6), with five replicates and ten chicks per replicate, from the combination of the factors: tomato flour (HT) (1% and 2%) and the sex of the birds (males and females). Relative liver weight, enzyme activities were evaluated: Alanine Aminotransferase (ALT), Aspartate Aminotransferase (AST), Alkaline Phosphatase, AST/ALT ratio, Total Protein, Albumin, Globulin, Urea, Uric Acid, Total and direct Bilirubin, in addition a study of abdominal and liver fat histopathology was performed by analysis of variance (ANOVA), using completely randomized design with 2x3 factorial arrangement; Tukey's multiple comparison of means test (p<0.05). The Chi-square test was used for the vacuole count. The experimental stage ended at 42 days of age, sacrificing 2 chicks per replicate, 10 chicks per treatment. Relative liver weight showed differences in unsupplemented males (T1) compared to males supplemented at 2% (T3) and unsupplemented females (T4) (P<0.05), total protein levels increased with the incorporation of HT in the diet, serum albumin concentration was reduced in birds supplemented with 2% HT in relation to unsupplemented birds and globulin levels increased as a function of HT dose in the diet (P<0.05). AST, ALT, AST/ALT ratio and alkaline phosphatase showed no differences between treatments (P>0.05). Serum urea and uric acid concentration was not affected by HT doses (P>0.05). There were differences in total and direct bilirubin levels (P<0.05). The area of adipocytes was greater in females than in males (P<0.05), the area of hepatocyte vacuoles did not show differences (P>0.05), however, the count of macro and microvacuoles showed a greater number of these in (T3) and (T6), (2% of HT) (P<0.05). It is concluded that the intake of tomato meal has a lipogenic effect on the liver tissue of broilers