"Implementacion de equipos para el fortalecimiento de la técnica de aglutinación microscópica en el laboratorio de leptospira y evaluación de las capas existentes en cuanto a su viabilidad y posibles contaminantes"

Abstract

The present degree work in the Community Development modality consisted of the implementation of equipment for the leptospira laboratory that is located in the agricultural sciences laboratory of the Technical University of Manabí in the Santa Ana canton, Lodana parish, this project had as purpose to provide laboratory equipment to obtain better results in the analysis of cultures and processing of samples for the investigation of leptospira. Based on the existing needs in the leptospira area, the project “Implementation of equipment to strengthen the Microscopic Agglutination Technique in the leptospira laboratory and evaluation of existing strains regarding their viability and possible contaminants”. In which criteria of those understood in the subject were used due to the needs that were identified throughout previous research studies in the area with undergraduate students. The implementation of the laboratory was carried out in order to satisfy the main needs of the leptospira laboratory and that serve at the general level of undergraduate and graduate students to carry out their research and be able to qualify as a Veterinarian and Magister. The purchase of equipment and devices such as the Type II Biological Safety Cabinet and Binocular Biological Microscope (Olympus CX33) was made where it allowed identifying this type of pathogens that is being reported in humans by contact with infected animals, so it is necessary its identification and serological diagnosis in domestic and wild animals. This area, being equipped with these equipment, allowed it to be independent for the preparation of culture media thanks to having a biosafety chamber typical of the area and being able to detect antibodies against leptospira through the technique of Microscopic Agglutination (MAT) with the provision of a biological microscope, which greatly strengthens this important area of research.