"Identificaciòn de leptospira patògena mediante el uso de PCR en bovinos del Centro de faenamiento Portoviejo, y su potencial riesgo de infecciòn al ser humano".

Abstract

Leptospirosis is an infectious-contagious disease of worldwide distribution, caused by bacteria of the genus Leptospira; tropical, subtropical, and humid climates favor its presentation and proliferation, this disease affects both domestic and wild animals and can even affect humans because it is a zoonosis, which causes a great impact on public health. The purpose of this research was to identify the presence of pathogenic leptospira using conventional PCR in bovine urine from the Portoviejo Municipal Slaughterhouse (CFMP), and to establish the potential risk of infection to humans who are in contact with these infected animals; 376 urine samples were collected from bovines from the slaughter line at the Portoviejo slaughterhouse; 10±1 daily samples were collected for a period of 40 days, for the processing of the samples in the laboratory, the samples were grouped 5 samples per day, obtaining 2 grouped daily samples obtaining a total of 75 samples to which a conventional PCR was performed using primer hap 1 that amplifies the gene lipL32 main outer membrane protein of pathogenic leptospira species, The study was able to detect 5.3% (4/75) at individual level and 10% (4/40) of days of positivity to the presence of pathogenic leptospira DNA in the study; HSe and HSp were obtained of 70% y 35% respectively, for positive and negative days, being days 7, 12, 15 and 20 those that presented a risk of infecting the people who perform the tasks, due to the presence of pathogenic leptospira DNA, which is why it is necessary to maintain the constant use of personal protective equipment in the personnel working in the CFMP slaughtering task.