THE COTTON (GOSSYPIUM HIRSUTUM. L), IS A VERY IMPORTANT FIBER CROP AND IS CONSIDERED THE BACKBONE OF THE ECONOMY OF MANY COUNTRIES AROUND THE WORLD. THE CULTIVATION OF COTTON FOR ITS OPTIMAL GROWTH AND DEVELOPMENT REQUIRES ADEQUATE AMOUNTS OF MACROS AND MICRONUTRIENTS, WHERE NITROGEN IS THE NUTRITIONAL ELEMENT MOST REQUIRED BY THIS SPECIES. THE MAIN OBJECTIVE OF THE PRESENT STUDY IS TO EVALUATE THE EFFECT OF FOUR DOSES OF NITROGEN SOURCES BASED ON BOVINE MANURE AND UREA, ON THE PHYSIOLOGICAL AND PRODUCTIVE BEHAVIOR OF COTTON CULTIVATION (GOSSYPIUM HIRSUTUM, L.), VARIETY DP ACALA-90. 8 TREATMENTS WERE EVALUATED (T1: BOVINE MANURE IN 50 KG OF N HA-1, T2: UREA IN 50 KG OF N HA-1, T3: BOVINE MANURE IN 100 KG OF N HA-1, T4: UREA IN 100 KG OF N HA-1, T5: BOVINE MANURE IN 150 KG OF N HA-1, T6: UREA IN 150 KG OF N HA-1, T7: BOVINE MANURE IN 200 KG IN N HA-1 AND T8: UREA IN 200 KG OF N HA-1), USING A COMPLETELY RANDOMIZED BLOCK DESIGN (DBCA), IN THREE REPETITIONS, TOTALING 24 EXPERIMENTAL UNITS.