"DIAGNÓSTICO DE MYCOBACTERIUM SPP. EN EL CENTRO FAENAMIENTO DEL CANTÓN SANTA ANA DE LA PROVINCIA DE MANABÍ"

Abstract

Bovine tuberculosis is an infectious and zoonotic disease, considered a notifiable disease, because it is widely distributed in cattle worldwide, causing economic losses in livestock production, trade restrictions, death of the animal and is a potential risk to human health. The main objective of this study was “To determine, through Ziehl-Neelsen staining, the presence of Mycobacterium spp. in the lymph nodes with lesions compatible with bovine tuberculosis, of the carcasses of cattle slaughtered in the slaughtering center of the Santa Ana canton and relate it to the variables under study. This research was carried out in 2 phases, the first was the field carried out in the slaughter center of the Santa Ana canton, analyzing the slaughtered cattle, and samples of lymph nodes and lungs that presented lesions typical of bovine tuberculosis were taken. data such as age, sex, and body condition, in the month of March, a period in which 87 animals arrived. The second phase consisted of the processing of the samples taken, where the most affected part of these nodes and lungs was sown in specific culture media for the growth of Mycobacterium spp., Ziehl-Neelsen staining was also performed to determine the presence of AFB (Acid Alcohol Resistant Bacilli). The results obtained were 15 animals that showed colony growth after the weeks of observation (it is worth mentioning that there was more colony growth in the Stonebrink culture medium than in the Ogawa Kudoh culture medium), these 15 animals with growth Ziehl-Neelsen staining was performed, where 5 animals were positive for Mycobacterium spp. of 44 bovines sampled: once these data were analyzed, the prevalence of this study was obtained, giving 11.36%, considered a high prevalence. And, on the other hand, the proportion of slaughtered cattle with typical bovine tuberculosis lesions was 50.6% (44/87). Based on the results obtained, the presence of AFB is concluded, which is a main characteristic of Mycobacterium spp., in the slaughtered cattle corresponding to the slaughter center of the Santa Ana canton. The macroscopic lesions were compatible with the typical lymph node lesions at the bovine tuberculosis.